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The Use of Standardized Reference Antigens in the Serogrouping of Streptococci
Author(s) -
DENT V. E.,
MARSH P. D.
Publication year - 1979
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1979.tb00846.x
Subject(s) - serotype , antiserum , antigen , microbiology and biotechnology , serology , streptococcus , biology , group a , streptococcus bovis , virology , medicine , antibody , bacteria , immunology , genetics , rumen , food science , fermentation
Streptococci isolated from the dental plaque of five animal species were identified by physiological and serological methods. Twenty‐nine strains of streptococci considered to resemble Streptococcus mitior or Strep. bovis on the basis of physiological data reacted with Lancefield group B or group K antisera respectively in tube precipitation tests. Further serological studies with standardized antigens from known serogroup B and K streptococci revealed that only three of these 29 isolates had been serogrouped accurately and carried the appropriate group antigen. Comparisons were made between the reactivity of the antisera produced by Difco and Wellcome Reagents with acid and autoclaved extracts of the strains. It was shown that the accuracy of serogrouping such isolates could be improved if the tests were made in a gel diffusion system that included a reference antigen.