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A Rapid Method for Purifying Bacterial Deoxyribonucleic acid
Author(s) -
GIBSON D. M.,
OGDEN I. D.
Publication year - 1979
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1979.tb00839.x
Subject(s) - pronase , dna , elution , chromatography , chemistry , rna , sepharose , microbiology and biotechnology , biochemistry , biology , trypsin , gene , enzyme
DNA was purified from bacterial cell lysates by treatment with RNA ase and Pronase followed by chromatography on a column of Sepharose 4B with standard saline citrate buffer as eluant. It has the same base composition as DNA prepared by‐Marmur's (1961) method.