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A Modification of Brilliant Green Agar for Improved Isolation of Salmonella
Author(s) -
WATSON D. C.,
WALKER A. P.
Publication year - 1978
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1978.tb04214.x
Subject(s) - salmonella , agar , brilliant green , tetrathionate , isolation (microbiology) , serotype , microbiology and biotechnology , antimicrobial , food science , chemistry , agar plate , chromatography , biology , bacteria , organic chemistry , genetics
Five organisms commonly found to be capable of growth on commercial Brilliant Green Agar (BGA) after enrichment in Muller‐Kauffman Tetrathionate broth (MKT) were tested for sensitivity to 18 antimicrobial agents. The sensitivities of two Salmonella serotypes to these agents were also tested. A combination of sulphacetamide (at 1.0 mg/ml) and mandelic acid (at 0.25 mg/ml) incorporated into BGA was found to give maximum recovery of salmonellas from MKT broth enrichment whilst giving maximum suppression of contaminating organisms. More importantly, this Antibiotic‐enriched Brilliant Green Agar (ABG) gave a lower incidence of false positive results when compared with commercial BGA. Increasing the incubation temperature from 35 to 43°C was found to accentuate the selectivity of ABG without inhibiting the growth of salmonellas. A total of 31 Salmonella serotypes were tested for their ability to grow on ABG at 43°C; all produced typical colonies.