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An Improved Electrophoretic Method for Identifying Antibiotics with Special Reference to Animal Tissues and Animal Feeding Stuffs
Author(s) -
SMITHER R.,
VAUGHAN D. R.
Publication year - 1978
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1978.tb00816.x
Subject(s) - antibiotics , micrococcus luteus , bacillus cereus , agarose , agarose gel electrophoresis , biology , agar , oxytetracycline , microbiology and biotechnology , identification (biology) , bacteria , biochemistry , staphylococcus aureus , botany , genetics , gene
Fifty antibacterial agents, mainly antibiotics, were examined by a high voltage electrophoretic technique which determined their migration distances in agar and agarose gels at both pH 6·0 and 8·0. Comparison of the different migration distances in the two gels formed the basis for identification. Bio‐autography, using Bacillus cereus var. mycoides or Micrococcus luteus , was used to visualize the antibiotics. The method was satisfactory for identifying many antibiotics in animal tissues and animal feeding stuffs, and was capable of distinguishing antibiotic activity from that of naturally occurring inhibitors often present in those materials. It has also been used to identify antibiotics in urine and pharmaceutical preparations, and a wider application in medical microbiology is indicated.