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Growth of Various Bacteria on Polycyclic Aromatic Hydrocarbons and N‐2–Fluorenylacetamide
Author(s) -
MARTINSEN CHARLENE S.,
ZACHARIAH PRINCE K.
Publication year - 1978
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1978.tb00810.x
Subject(s) - pyrene , chemistry , metabolite , bacteria , carcinogen , polycyclic aromatic hydrocarbon , chromatography , environmental chemistry , metabolism , organic chemistry , biochemistry , biology , genetics
Several heterotrophic bacteria grew in nutrient broth medium containing high concentrations (10 −5 M) of the potent carcinogenic compounds, benz[a]pyrene, 3‐methylcholanthrene or N‐2–fluorenylacetamide. They were capable of metabolizing the polycyclic aromatic hydrocarbons to smaller molecules and utilized these compounds as the sole source of carbon and energy. Identification of the metabolites formed from benz[a]pyrene revealed that the major metabolite (3‐hydroxybenz[a]pyrene) of mammalian systems did not accumulate in any of these cultures when the standard fluorometric assay was used. Benz[a]pyrene metabolism profiles with high‐pressure liquid chromatography also exhibited no accumulation of any metabolites (hydroxy, quinone and diol derivatives).