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Influence of the Growth Phase and Fimbriae on the Gradient Elution of Salmonella typhimurium from DEAE‐cellulose
Author(s) -
HALL A. N.,
HOGG S. D.,
PHILLIPS G. O.
Publication year - 1978
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1978.tb00793.x
Subject(s) - fimbria , stationary phase , elution , phase (matter) , chemistry , gradient elution , phosphate buffered saline , cellulose , exponential growth , phosphate , chromatography , biochemistry , escherichia coli , high performance liquid chromatography , physics , organic chemistry , gene , quantum mechanics
Initial investigations showed that certain populations of Sytyphimurium cells could be resolved into three fractions by eluting the cells from DEAE‐cellulose with a NaCl gradient in 0·05 Mphosphate buffer (pH 6·0). The three fractions were eluted with 0, 60 and 190 mmol/l NaCl. The results obtained with two strains indicate that non‐fimbriate, exponential phase cells; fimbriate, stationary phase cells; and non‐fimbriate, stationary phase cells are eluted with 0, 60 and 190 mmol/l NaCl in 0·05 M‐phosphate buffer (pH 6·0), respectively. These conclusions appear to be consistent with current views on the effects of fimbriae and the exponential growth phase in reducing the electrical charge carried by bacterial cells.