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Sodium Dodecyl Sulphate‐Polyacrylamide Gel Electrophoresis of Polypeptides from Bacteraides melaninogenicus
Author(s) -
SWINDLEHURST C.A.,
SHAH H. N.,
PARR C. W.,
WILLIAMS R. A. D.
Publication year - 1977
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1977.tb00757.x
Subject(s) - subspecies , polyacrylamide gel electrophoresis , electrophoresis , biology , gel electrophoresis , strain (injury) , microbiology and biotechnology , antiserum , serotype , sodium , biochemistry , chemistry , enzyme , genetics , zoology , anatomy , antibody , organic chemistry
1 Sodium dodecyl sulphate‐polyacrylamide gel electrophoresis has been applied to proteins extracted from 32 strains of Bacteroides melaninogenicus . 2 Strains of subspecies melaninogenicus gave similar electrophoretic patterns which differed from those of strains outside the subspecies. 3 Strains of subspecies intermedius serotype C were similar to one another in electrophoretic pattern, and were different from strains outside the subspecies and from one strain of subspecies intermedius of serotype C1. 4 Greater variation was observed between asaccharolytic than between saccharolytic strains. 5 The group within subspecies asaccharolyticus with low G + C content stained poorly and showed few bands. 6 The group within subspecies asaccharolyticus with high G + C content showed interstrain differences but none would be confused with strains of the saccharolytic subspecies. The strain that did not react with type B antiserum was electrophoretically different from those strains that did. 7 The asaccharolytic Lev type strains form a separate group based on both electrophoretic and physiological criteria.