Premium
Observations on Pre‐enrichment for Isolating Salmonellas from Sewage Polluted Natural Water Using Muller‐Kauffmann Tetrathionate Broth Prepared with Fresh and Desiccated Ox Bile
Author(s) -
HARVEY R. W. S.,
PRICE T. H.
Publication year - 1977
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1977.tb00733.x
Subject(s) - tetrathionate , brilliant green , sewage , salmonella , food science , clean water , biology , isolation (microbiology) , agar , chemistry , microbiology and biotechnology , chromatography , bacteria , environmental science , environmental engineering , waste management , genetics , organic chemistry , engineering
Desiccated bile (Oxoid L50) was substituted for fresh bile obtained from the abattoir in Muller‐Kauffmann Tetrathionate Broth. Two versions of the enrichment medium were prepared, one with desiccated bile the other with fresh bile. The efficiency of these two media was compared using 25 ml quantities of sewage polluted natural water as inocula. Direct enrichment and preenrichment techniques were used. With direct enrichment the Muller‐Kauffmann Tetrathionate Broth prepared from fresh bile was more efficient for salmonella isolation. With the pre‐enrichment technique using preliminary culture of the water sample in buffered peptone water prior to enrichment, there was no significant difference between the efficiency of the two versions of tetrathionate. Comparison of direct enrichment with the pre‐enrichment method for isolating salmonellas from sewage polluted water clearly demonstrated the advantages of pre‐enrichment. This would certainly have a bearing on the quantitative estimation of salmonellas in polluted water and might increase the number of serotypes isolated from a sample divided into subsamples.