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Immunocytochemical Localization of Spore Specific Antigens in Ultrathin Sections
Author(s) -
SHORT J. A.,
WALKER P. D.,
HINE P.,
THOMSON R. O.
Publication year - 1977
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1977.tb00724.x
Subject(s) - cytoplasm , dipicolinic acid , bacillus subtilis , antigen , spore , biology , microbiology and biotechnology , bacillus cereus , endospore , immunocytochemistry , bacterial outer membrane , gene , genetics , bacteria , escherichia coli , endocrinology
It has been demonstrated, using immunocytochemical techniques, that individual spore antigens are synthesized in discrete compartments of the bacterial cell. An outer layer of bacterial spores, demonstrable ultrastructurally as a distinct exosporium in Bacillus cereus or as an outer tight‐fitting sporecoat in B. subtilis , is synthesized in the cytoplasm of the mother cell. Conversely, the layers of the inner sporecoat antigens are synthesized in the forespore compartment and in association with the forespore membranes. Different layers of the sporecoat are thus synthesized in separate morphological areas and are presumably under different genetic control. Immunocytochemical techniques indicate that dipicolinic acid is found in association with the sporecore.