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Feasibility of Thin Layer Chromatography as an Inexpensive Alternative to Gas‐Liquid Chromatography for the Identification of Some Anaerobic Gram Positive Non‐sporing Rods
Author(s) -
PALASUNTHERAM C.,
DRUCKER D. B.,
TUXFORD A. F.
Publication year - 1977
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1977.tb00715.x
Subject(s) - chromatography , gas chromatography , lactobacillus , thin layer chromatography , chemistry , fermentation , organic chemistry
1 Thin‐layer chromatography (TLC) was employed as an alternative to gas‐liquid chromatography (GLC) for the analysis of typical fermentation end‐products of anaerobic Gram positive non‐sporing bacteria. The inexpensive TLC method succeeded in differentiating Actinomyces, Bifidobacterium, Lactobacillus and Propionibacterium . 2 Ether extraction of supernatant was found to be unnecessary; volatile and non‐volatile acids were separated simultaneously, with consequent saving of time.