Premium
Proximate Analyses of Rumen Fluid Preparations Used in Microbiological Media
Author(s) -
INGLEDEW W. M.
Publication year - 1977
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1977.tb00700.x
Subject(s) - rumen , composition (language) , escherichia coli , bacteria , food science , ammonia , nutrient , chemistry , volatile fatty acids , carbohydrate , biochemistry , biology , fermentation , organic chemistry , genetics , philosophy , linguistics , gene
Proximate analyses of clarified rumen fluid No. 1 (CRF1) and No. 2 (CRF2). and of an aqueous extract of Escherichia coli cells are reported. Differences in the procedures for preparing CRF2 and CRF1 from the same batch of whole rumen fluid were reflected by increased levels of carbohydrate, protein and total nitrogen in CRF2. Variations in the composition of CRF2 with time after feeding are also reported. Results showed CRF2 to be an inconsistent and non‐reproducible medium supplement. The extract of E. coli cells contained very low levels of volatile fatty acids and ammonia and thus by itself could not replace CRF2 in medium for many species of rumen bacteria. It could be used as an alternative to CRF2 in media where more consistent and reproducible composition was required if the medium were supplemented with defined mixtures of nutrients known to be required by rumen bacteria.