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Rapid Techniques for the Examination of Bacterial Citrate Synthases
Author(s) -
HARFORD S.,
JONES DOROTHY,
WEITZMAN P. D. J.
Publication year - 1976
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1976.tb00659.x
Subject(s) - citrate synthase , size exclusion chromatography , biochemistry , chromatography , chemistry , bacteria , agar , filtration (mathematics) , agar plate , enzyme , computational biology , biology , mathematics , genetics , statistics
A method has been devised for evaluating easily and rapidly the effect of inhibitors on the activity of citrate synthase in situ in bacteria grown on agar plates. Additionally, considerable reduction in the size of the gel filtration column employed to estimate the molecular weight of citrate synthase enables such a determination to be made in a much shorter time. These two techniques make it much easier to exploit the properties of different bacterial citrate synthases as an additional taxonomic tool in bacteriological laboratories.