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Rapid Confirmation of Suspect Salmonella Colonies by Use of the Minitek System in Conjunction with Serological Tests
Author(s) -
COX N. A.,
MERCURI A. J.
Publication year - 1976
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1976.tb00650.x
Subject(s) - salmonella , microbiology and biotechnology , serotype , agar , incubation , biology , serology , lactose , antiserum , maltose , enterobacteriaceae , food science , sucrose , bacteria , antigen , escherichia coli , biochemistry , antibody , immunology , genetics , gene
Colonies of 40 members of the Enterobacteriaceae family (26 Salmonella serotypes and 14 other organisms) were picked from selective agar plates and inoculated into Minitek inoculum broth (BBL) and then onto Minitek discs of dextrose, lactose, sucrose, mannitol, maltose, dulcitol, lysine and H 2 S. After incubation for 6 h, the inoculum broth was tested with salmonella Poly O and after 24 h with salmonella Poly H antisera. The results of the biochemical tests were read after 24 h incubation. With this procedure, all the salmonella cultures used in this study were confirmed as salmonella and differentiated from all the other organisms, which were rejected. This procedure provides an alternative to the time consuming conventional procedures for the biochemical and serological confirmation of suspect salmonella colonies on selective agar plates.