Premium
The Cultivation of the Rumen Ciliate Entodinium longinucleatum
Author(s) -
OWEN R. W.,
COLEMAN G. S.
Publication year - 1976
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1976.tb00641.x
Subject(s) - rumen , biology , protozoa , ciliate , starch , dilution , inoculation , zoology , serial dilution , bacteria , microbiology and biotechnology , food science , horticulture , ecology , fermentation , medicine , physics , alternative medicine , pathology , thermodynamics , genetics
1 Two isolates of Entodinium longinucleatum have been cultured in vitro for at least six months in the presence of bacteria on a reduced buffered salts medium with the daily addition of starch grains and ground dried grass. 2 Cultures of Entodinium longinucleatum were normally maintained by dilution, twice each week, with an equal volume of fresh medium, but the protozoa would also grow from a 2.5% inoculum with a mean generation time of 1.3 days.