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Oxidation of Arsenite by a Soil Isolate of Alcaligenes
Author(s) -
OSBORNE F. H.,
EHRLICH H. L.
Publication year - 1976
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1976.tb00633.x
Subject(s) - arsenite , arsenate , alcaligenes faecalis , oxidizing agent , chemistry , oxygen , cytochrome , arsenic , alcaligenes , enzyme , inorganic chemistry , nuclear chemistry , biochemistry , biology , organic chemistry , bacteria , pseudomonas , genetics
A strain of Alcaligenes , isolated from soil and grown in nutrient broth in the presence of arsenite, possessed the ability to oxidize arsenite to arsenate. Washed cell suspensions consumed one‐half mol of oxygen/mol of arsenite and produced arsenate. The optimum pH for arsenite oxidation was 7.0. The K m for arsenite was 1.5 × 10 ‐4 M and V max was 6.7 μl of oxygen/min. The arsenite‐oxidizing enzyme system was induced by growth in arsenite. Response of the arsenite‐oxidizing enzyme system to respiratory inhibitors suggested that electrons resulting from arsenite oxidation by an oxido‐reductase with a bound flavin are transferred via cytochrome c and cytochrome oxidase to oxygen. The presence of the cytochromes in crude extract was confirmed by spectral measurements.