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A Simple Procedure for Screening of Salmonellae using a Semi‐Solid Enrichment and a Semi‐Solid Indicator Medium
Author(s) -
CHAU P. Y.,
HUANG C. T.
Publication year - 1976
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1976.tb00632.x
Subject(s) - citrobacter freundii , salmonella , triphenylmethane , enterobacter cloacae , chromatography , citrobacter , enterobacter , novobiocin , chemistry , microbiology and biotechnology , direct agglutination test , biology , bacteria , enterobacteriaceae , biochemistry , escherichia coli , organic chemistry , genetics , immunology , serology , antibody , gene , antibiotics
The incorporation of triphenylmethane dyes and divalent cations in a semi‐solid medium in conjunction with a low pH retarded the motility of most enteric bacteria except for Salmonella, Enterobacter cloacae and Citrobacter freundii . The migration of the latter two species in the semi‐solid medium, however, could be selectively inhibited by the incorporation of novobiocin to the medium and by incubation at 41 °C. Based on these observations, a semi‐solid enrichment medium was developed and used successfully in a Salmonella screening procedure in which a semi‐solid indicator medium was used as the first and a slide agglutination test as the second screen. Clinical laboratory evaluation of the procedure showed an increase of 92% in the frequency of positive isolations, with no false positives, as compared with a conventional multi‐step isolation procedure.