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Application of Flow Microcalorimetry to Analytical Problems: the Preparation, Storage and Assay of Frozen Inocula of Saccharomyces cerevisiae
Author(s) -
BEEZER A. E.,
NEWELL R. D.,
TYRRELL H. J. V.
Publication year - 1976
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1976.tb00620.x
Subject(s) - isothermal microcalorimetry , yeast , liquid nitrogen , saccharomyces cerevisiae , chromatography , chemistry , microbiology and biotechnology , biology , biochemistry , thermodynamics , physics , organic chemistry , enthalpy
A simple procedure for the freezing of large batches of yeast inocula (up to 150 samples) for storage at liquid nitrogen temperatures with the retention of high viability after thawing is described. The recovered frozen cells were examined using a flow microcalorimetric procedure which enables a rapid determination of viability. The application of such inocula to antibiotic assays and growth studies is discussed.