Premium
Gradient Elution of Salmonella typhimurium and Escherichia coli Strains from a DEAE‐Cellulose Column
Author(s) -
HALL A. N.,
HOGG S. D.,
PHILLIPS G. O.
Publication year - 1976
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1976.tb00618.x
Subject(s) - elution , escherichia coli , salmonella , chromatography , chemistry , agar , gradient elution , yield (engineering) , cellulose , bacteria , microbiology and biotechnology , biochemistry , biology , high performance liquid chromatography , materials science , genetics , metallurgy , gene
1 A good separation of cells of Salmonella typhimurium ATCC 14028 and Escherichia coli NCIB 8134, which had been grown on nutrient agar for 18 h at 37 °C, was obtained by elution from a column of Whatman DE‐32 cellulose with a linear NaCl gradient in 0.05 M‐phosphate at pH 6.0. 2. The identity of the separated fractions was established by comparison with the elution patterns of the individual bacteria and by means of diagnostic tests. 3 Salmonella typhimurium cells separated into two fractions under these conditions. 4 The yield of cells recovered from a column was considerably increased by inclusion of 0.05% (w/v) Tween 80 in the eluant system.