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Ultrasonication—An Enrichment Technique for Microcyst‐forming Bacteria
Author(s) -
SUTHERLAND I. W.
Publication year - 1976
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1976.tb00617.x
Subject(s) - isolation (microbiology) , myxobacteria , bacteria , microbiology and biotechnology , sonication , azotobacter , biology , chemistry , botany , chromatography , genetics
Brief ultrasonic treatment of samples of bark, soil or water containing fruiting myxobacteria yields higher isolation rates than conventional procedures when aliquots are applied to solid media. Many normal contaminants, including nematodes, are destroyed and representative strains of most genera of Myxobacteria and Sporocytophaga have been isolated in this way. The technique can also be applied to the isolation of cyst‐forming Azotobacter spp. and may be suitable for cyst‐forming Methanobacterium spp. A further application is in the comparison of bacillary and resting forms in natural environments.