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An Indirect Radiolabelled Antibody Staining Technique for the Rapid Detection and Identification of Bacteria
Author(s) -
BENBOUGH J. E.,
MARTIN K. L.
Publication year - 1976
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1976.tb00604.x
Subject(s) - serratia marcescens , staining , bacillus subtilis , antibody , francisella tularensis , bacteria , microbiology and biotechnology , biology , globulin , chemistry , gamma globulin , chromatography , escherichia coli , biochemistry , immunology , genetics , gene , virulence
Small numbers of Bacillus subtilis var. niger spores, Serratia marcescens and Francisella tularensis can be detected and identified in an hour using an indirect radiolabelled antibody staining technique with homologous rabbit antibacterial antibody and radiolabelled goat anti‐rabbit globulin. Tritium is better than 125 I, 14 C and 35 S for labelling the globulin. The reagent obtained is sensitive and adequately stable.