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Use of a Coulter Counter to Detect Discrete Changes in Cell Numbers and Volume During Growth of Escherichia coli
Author(s) -
SMITHER R.
Publication year - 1975
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1975.tb00557.x
Subject(s) - coulter counter , escherichia coli , volume (thermodynamics) , bacterial growth , bacteriuria , cell counting , chromatography , chemistry , biology , microbiology and biotechnology , bacteria , cell , biochemistry , urine , thermodynamics , physics , genetics , cell cycle , gene
Escherichia coli could be detected at a concentration of 10 5 cells/ml. Growth curves of the organism grown in filtered tryptone soya broth were made by taking viable and Coulter counts. Both curves were similar in shape and varied little during 10 h growth. Cell volume and volume distribution were seen to vary considerably however, volume reaching a stable minimum value of 0·61 μm 3 after 8 h. The Coulter Counter was considered to be potentially useful for detecting significant bacteriuria.