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The Preservation of Bacteria and Fungi on anhydrous Silica Gel: an Assessment of Survival over Four years
Author(s) -
TROLLOPE D. R.
Publication year - 1975
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1975.tb00511.x
Subject(s) - bacilli , bacteria , silica gel , anhydrous , microbiology and biotechnology , microorganism , biology , skimmed milk , chemistry , food science , chromatography , organic chemistry , genetics
The preservation method of Perkins (1962) using suspensions in skim‐milk was used to preserve 33 bacteria and 22 fungi on anhydrous silica gel. During storage at room temperature, 64% of the bacteria and 77% of the fungi survived 1 year or more. Storage at 4° often increased the survival period c. 2‐ to 3‐fold: 73% of the bacteria and all 12 of the fungi tested at 4° survived > 1 year. At the last testing, 60% of the bacteria and 36% of the fungi were still viable after storage at 4° for periods between 3 and 4 years. The Gram positive bacilli tended to survive the silica gel preservation process better than most Gram negative bacilli. Some factors influencing survival after preservation on silica gel are discussed; the results support the use of a closed storage tube.