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The Formation, General Characteristics and Production of α‐Amylase in Heterocaryons and Diploids of Aspergillus oryzae
Author(s) -
Thorbek Lene,
Eplov P.
Publication year - 1974
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1974.tb00480.x
Subject(s) - conidium , ploidy , aspergillus oryzae , biology , mutant , auxotrophy , strain (injury) , amylase , horticulture , yield (engineering) , botany , genetics , food science , gene , physics , enzyme , fermentation , biochemistry , anatomy , thermodynamics
S ummary . Heterocaryons and diploids from Aspergillus oryzae were investigated with respect to nuclear number/conidium and to conidial size. Heterocaryons usually had larger conidia and more nuclei/conidium than diploids and the haploid parent mutants. Diploids contained significantly fewer nuclei/conidium than haploids. However, they could not be distinguished from haploids by measurement of conidial size. The strains were examined for the production of α‐amylase. All auxotrophic mutants produced less α‐amylase than the prototrophic wild type. Heterocaryons gave yields which were intermediate between that of their parent mutants or the same as the best producing parent. Diploids which produced more α‐amylase than the best producing parent strain were synthesized. The highest yield from a diploid was of the same order of magnitude as the yield from the wild type.