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Rapid Screening for the Isolation of Mutants of Aspergillus nidulans with Increased Penicillin Yields
Author(s) -
Ditchburn P.,
Giddings B.,
Macdonald K. D.
Publication year - 1974
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1974.tb00476.x
Subject(s) - aspergillus nidulans , penicillin , spore , agar , bacillus subtilis , microbiology and biotechnology , conidium , strain (injury) , isolation (microbiology) , agar plate , aspergillus , mutant , biology , inoculation , chemistry , antibiotics , bacteria , botany , biochemistry , horticulture , genetics , anatomy , gene
S ummary . After UV treatment conidia of a strain of Aspergillus nidulans were plated on an agar medium. The survivors gave rise to individual colonies which were inoculated separately on agar discs and incubated. The discs were then transferred to biological assay plates seeded with spores of a strain of Bacillus subtilis sensitive to penicillin. Using this primary screening method, mutants have been isolated which, when tested later in shake flask cultures, gave larger penicillin yields than the parent strains.