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Analysis of the Intracellular Amino Acid Pool and Proteins from Whole Cells of Rhizobium japonicum
Author(s) -
Lillich T. T.,
Elkan G. H.
Publication year - 1973
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1973.tb04108.x
Subject(s) - amino acid , peptidoglycan , alanine , biochemistry , bacterial cell structure , lysine , intracellular , cell wall , bacteria , biology , glutamate dehydrogenase , chemistry , glutamate receptor , receptor , genetics
. The amino acid composition of proteins from whole cells of Rhizobium japonicum was determined. The proteins contain only those amino acids commonly associated with bacteria and in ratios approximately equal to those which have been reported in other bacterial genera. Analysis of the intracellular amino acid pool showed relatively large amounts of alanine, glutamate and lysine. These amino acids are normally associated with the peptidoglycan layer of bacterial cell walls leading to speculation that the pool observed was for the synthesis of cell wall precursors rather than for general protein synthesis. The high levels of alanine relative to the other amino acids can also be explained by the operation of alanine dehydrogenase, (E.C. 1.4.1.1) which is an assiniilatory enzyme for ammonia.