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The Affinity for Methane and Methanol of Mixed Cultures Grown on Methane in Continuous Culture
Author(s) -
Wilkinson T. G.,
Harrison D. E. F.
Publication year - 1973
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1973.tb04107.x
Subject(s) - methanol , methane , chemistry , substrate (aquarium) , affinities , anaerobic oxidation of methane , nuclear chemistry , biochemistry , biology , organic chemistry , ecology
. Pure cultures of Hyphomicrobium sp. had a high affinity for methanol ( K m = 8·33 × 10 −6 M) and the maximum respiration rate was c. 5 times higher than that of a mixed culture in which a methane‐utilizing pseudomonad predominated ( K m = 2·94 × 10 −2 M). From a comparison of the substrate affinities, a culture of the methane‐utilizing organism would be expected to produce levels of methanol high enough to inhibit methane oxidation and the Hyphomicrobium sp. would be able to scavenge this methanol.