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Interaction of Glutaraldehyde with Spheroplasts of Escherichia coli
Author(s) -
Munton T. J.,
Russell A. D.
Publication year - 1973
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1973.tb04093.x
Subject(s) - spheroplast , glutaraldehyde , escherichia coli , lysis , chemistry , sodium bicarbonate , tonicity , microbiology and biotechnology , biochemistry , biology , chromatography , organic chemistry , gene
. Glutaraldehyde, in the presence or absence of sodium bicarbonate, caused an increase in the optical density of spheroplasts of Escherichia coli. The aldehyde, particularly at alkaline pH, prevented lysis of spheroplasts when these were suddenly exposed to a hypotonic environment. It is proposed that the stabilization which occurs results from the crosslinking activity of glutaraldehyde on the cell envelope of E. coli.