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Further Studies on a Serum Nonantibody α Globulin with Antistaphylococcal Activity
Author(s) -
Yotis W. W.,
Lentino J. R.
Publication year - 1973
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1973.tb04082.x
Subject(s) - antiserum , chemistry , globulin , haptoglobin , fibrinogen , blood proteins , lysin , gamma globulin , biochemistry , antibody , biology , immunology , bacteriophage , escherichia coli , gene
S ummary . Previous work in this laboratory indicated that normal human serum contains a pseudoglobulin which inhibited the growth and respiration of staphylococci. Studies with a variety of serum fractions suggest that antibacterial agent (ABA) and α globulin alone possess the antibacterial properties which distinguish them from all other fractions tested. ABA has been prepared from unfiltered and Seitz filtered (to remove the β lysin) sera, platelet‐rich plasmas of humans, rabbits, guineapigs and rats and assayed by a sensitive rapid radioisotopic technique. ABA is widely distributed among animal sera and, in contrast to the human ABA, requires a 2–4 fold increase in the concentration of coagulase necessary to demonstrate a comparable reversal of the antirespiratory effect. Immunological studies confirmed the nonantibody nature of ABA. ABA did not react with antisera to IgA, IgG, IgM, human β lipoprotein, haptoglobin, thyroglobulin, C‐reactive protein, ceruloplasmin, fibrinogen or antiserum to β lysin obtained from other workers. Of the various serum fractions tested only á globulin had any detectable antistaphylococcal activity.