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New Culture Conditions for Clostridium histolyticum leading to Production of Collagenase of High Specific Activity
Author(s) -
Takahashi S.,
Seifter S.
Publication year - 1972
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1972.tb03746.x
Subject(s) - collagenase , protease , chemistry , clostridium , biochemistry , microbiology and biotechnology , biology , enzyme , bacteria , genetics
S ummary : New culture conditions are described for the growth of Clostridium histolyticum allowing for the production of collagenase (E.C.3.4.4.19) in a highly purified form, free of general protease activity. The conditions include the use of a mixture of sodium bisulphite‐oxidized sodium thioglycollate to provide the reducing environment within an otherwise standard culture medium. Also, new modifications in the preparation of collagenase A are reported.