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A Comparison of Methods for the Enrichment of Salmonellae from Sheep Faeces
Author(s) -
Grau F. H.,
Smith M. G.
Publication year - 1972
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1972.tb03734.x
Subject(s) - tetrathionate , mannitol , selenium , feces , incubation , salmonella , brilliant green , biology , food science , cystine , chemistry , microbiology and biotechnology , bacteria , biochemistry , enzyme , genetics , organic chemistry , cysteine
S ummary : A 10‐tube MPN technique was used to test the efficiency of nutrient, tetrathionate, mannitol‐selenite and mannitol‐selenite‐cystine broths as enrichment media for detecting salmonellae. Small numbers of broth grown salmonellae could be detected in all 4 media in the presence of 5% of sheep faeces. In naturally infected sheep faeces small numbers of salmonellae were not detected with either nutrient or tetrathionate broths. With mannitol‐selenite the sensitivity of salmonella detection increased with both incubation temperature (37–43°) and the addition of faeces. The most sensitive and reliable medium for detecting salmonellae in naturally infected sheep faeces was mannitol‐selenite‐cystine broth. Neither incubation temperature (37–43°) nor the addition of faeces had a statistically significant effect on its sensitivity.