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The Semi Large‐scale Production, Extraction, Purification and Properties of an Antibiotic Produced by Bacillus licheniformis Strain 2725
Author(s) -
Woolford M. K.
Publication year - 1972
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1972.tb03694.x
Subject(s) - bacillus licheniformis , strain (injury) , extraction (chemistry) , antibiotics , microbiology and biotechnology , chemistry , staphylococcus aureus , bacteria , bacillus subtilis , food science , chromatography , biology , genetics , anatomy
S ummary : Production, extraction, purification and properties of an antibiotic produced by Bacillus liclieniformis , strain 2725 are described. Extraction efficiency was c. 50% with 8–10 g of product/350 1 batch culture, the product having an activity of 2000 units/mg (0·5 μg/ml) against Staphylococcus aureus Paler. The antibiotic was a peptide, was active against Gram positive and negative bacteria; it was stable over a wide pH range, its bactericidal activity was enhanced by serum, but it had a high toxicity and was haemolytic.