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Fluorescent Spectrophotometry in the Identification of Bacteria
Author(s) -
Ginell R.,
Feuchtbaum Regina J.
Publication year - 1972
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1972.tb03670.x
Subject(s) - acridine orange , fluorescence , excitation wavelength , wavelength , orange (colour) , spectrophotometry , chemistry , photochemistry , analytical chemistry (journal) , optics , chromatography , physics , biochemistry , apoptosis
S ummary : Preliminary results on a technique for identifying bacteria by spectrophoto‐fluorometry are presented. The 3 parameters involved, emission wavelength, excitation wavelength and intensity, are used to produce a topographic map characteristic of the fluorescence of the chemical bonds. Enhancement of the differences between species is possible using dyes such as acridine orange. Various improvements in the technique are presented.