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A Rapid, Inexpensive Bacterial Count Technique using Agar Droplets
Author(s) -
Sharpe A. N.,
Kilsby D. C.
Publication year - 1971
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1971.tb02303.x
Subject(s) - serial dilution , agar , plate count , incubation , chromatography , agar plate , viable count , chemistry , bacteria , biology , medicine , biochemistry , alternative medicine , pathology , genetics
Summary. A simple method is described for obtaining viable counts of aerobic bacteria. Dilutions are prepared in molten agar and colonies allowed to form in droplets (0·1 ml) of appropriate dilutions. Samples can be processed c. 3 times faster by this technique than with a standard pour plate method: it reduces costs, work and incubation time. Counts obtained with the droplets compare favourably with those obtained by pour plates.