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A Colorimetric Bioassay Method for Colicins
Author(s) -
Shan R.,
Hedges A. J.
Publication year - 1970
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1970.tb02234.x
Subject(s) - bioassay , colicin , chromatography , chloride , chemistry , simplicity , biology , escherichia coli , biochemistry , physics , ecology , organic chemistry , quantum mechanics , gene
Summary. A technique for the comparative assay of colicin samples is described. Relative potencies are estimated by a modification of the well known survivor count method, in which the lethal potential of samples is measured indirectly by colorimetric determination of their effect on the capacity of a sensitive indicator culture to reduce 2,3,5‐triphenyltetrazolium chloride under specified conditions. The method has (i) simplicity of operation, (ii) a wide range of application, (iii) rapidity of execution, (iv) high sensitivity, (v) good precision.