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The Dynamics of Decane and Glucose Utilization by a Pseudomonas sp. in Batch and Chemostat Cultures under Controlled Dissolved Oxygen Tensions
Author(s) -
Maclennan D. G.,
Pirt S. J.
Publication year - 1970
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1970.tb02211.x
Subject(s) - chemostat , decane , dilution , carbon fibers , biomass (ecology) , oxygen , chemistry , carbon source , nitrogen , environmental chemistry , chromatography , organic chemistry , biology , bacteria , biochemistry , ecology , materials science , genetics , physics , composite number , composite material , thermodynamics
Summary. A Pseudomonas sp. was grown in chemostat culture with either decane or glucose as sole carbon source. At a dilution rate of 0·1 h −1 the biomass concentration was directly proportional to the decane supplied up to a concentration of 8·2 g/l, of which 5·3 g were utilized and the remainder lost as vapour. The maximum biomass concentration was limited to 3 g of bacterial dry wt/1 by what appeared to be an inhibitory product. When the culture was grown under different controlled dissolved oxygen tensions (DOT) at a dilution rate of 0·1 h −1 with nitrogen‐limited growth the following observations were made: (1) the growth limiting DOT was 1·0‐3·0 mmHg with decane, and < 1·0 mmHg with glucose as carbon source; (2) to obtain the maximum ratio of oxygen uptake: carbon source uptake the DOT needed to be c . 6·0 mmHg: at lower values the degree of oxidation of products decreased; (3) with decane an appreciable proportion of the decane carbon utilized ( c. 60%) appeared in unidentified products other than biomass and CO 2 .