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The Use of Sulphite‐containing Media in the Isolation of Clostridium welchii
Author(s) -
Mead G. C.
Publication year - 1969
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1969.tb00984.x
Subject(s) - clostridia , isolation (microbiology) , clostridium perfringens , agar , clostridium , food science , microbiology and biotechnology , chemistry , biology , bacteria , genetics
Summary An investigation was made of cultural conditions affecting the blackening of colonies due to sulphite reduction by Clostridium welchii and several other clostridia in the sulphite‐polymyxin‐sulphadiazine agar of Angelotti et al. (1962). Reducing conditions in the medium were more critical for blackening than for growth, and a simple, modified isolation method for Cl. welchii is described which provides optimal conditions for both processes.