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A Selective Medium for the Isolation of Agrobacterium Species
Author(s) -
Clark A. G.
Publication year - 1969
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1969.tb00982.x
Subject(s) - agrobacterium , isolation (microbiology) , inoculation , biology , liquid medium , lactose , transformation (genetics) , bacteria , microbiology and biotechnology , chemistry , botany , horticulture , food science , chromatography , biochemistry , genetics , gene
Summary A defined salts medium containing lactose and MnSO 4 as selective agents is described. This medium inhibits nearly all organisms of the soil microflora except Agrobacterium spp. against which it has no repressive or mutagenic effect. By using a modified test for 3‐ketolactose production together with plant inoculation, qualitative and quantitative assays for agrobacteria are possible.