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Medium Volume and the Survival of Serratia marcescens
Author(s) -
Domey R. G.,
Saurino V. R.,
Simon G. T.
Publication year - 1969
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1969.tb00964.x
Subject(s) - serratia marcescens , facultative , volume (thermodynamics) , petri dish , biology , colony forming unit , agar , microbiology and biotechnology , zoology , bacteria , escherichia coli , ecology , physics , thermodynamics , biochemistry , gene , genetics
Summary Experiments were designed to test the null hypothesis that volume of agar medium in 100 mm Petri dishes has no influence on the colony count of the facultative organism, Serratia marcescens. With a constant inoculum in 9, 12, 15 and 18 ml volume of medium the surface counts were statistically equivalent, but the subsurface counts increased as a direct function of volume variation. Over the range studied, at the P = 0.95 level, the null hypothesis was accepted for surface colony counts but was rejected for subsurface colony counts.