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Studies Arising from Observations of Osmophilic Yeasts by Phase Contrast Microscopy
Author(s) -
Scarr M. Pamela
Publication year - 1968
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1968.tb00401.x
Subject(s) - sugar , phase contrast microscopy , sucrose , chemistry , raw material , food science , contrast (vision) , biochemistry , organic chemistry , physics , optics , artificial intelligence , computer science
SUMMARY Osmophilic yeasts growing in concentrated sugar solutions vary in cell density with age and activity. Microscopic observations with phase contrast by Barer & Joseph's (1958) technique have confirmed this with all strains examined. Examination of syrups containing a culture of Saccharomyces rouxii indicates that growth is limited to these high concentrations owing to the physiological dryness of the habitat. However, some growth does take place, even in the film of molasses surrounding the sucrose crystal in stored raw sugar. At c. 60% (w/w) of dissolved solids it is possible to identify active cells microscopically. Therefore the examination of raw sugar consignments for such cells is a rapid way of indicating whether the sugar is likely to deteriorate on storage.