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Combined Effect of Water Activity, pH and Temperature on the Growth of Clostridium botulinum from Spore and Vegetative Cell Inocula
Author(s) -
BairdParker A. C.,
Freame Barbara
Publication year - 1967
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1967.tb00320.x
Subject(s) - spore , clostridium botulinum , germination , vegetative reproduction , spore germination , glycerol , biology , microbiology and biotechnology , chemistry , horticulture , biochemistry , toxin
SUMMARY The initiation of vegetative growth by spores in Reinforced Clostridial Medium (RCM) adjusted to different water activities (a w ) by the addition of NaCl or glycerol does not depend solely on the a w of the medium; minimum a w at which spores of Cl. botulinum types A, B and E could initiate growth were higher in the media containing NaCl. The minimum a w at which vegetative cell inocula could grow were similar to those at which spore inocula could initiate growth. The combined effect of lowering the pH and a w of the medium was to increase the minimum at which spores of types A and E could germinate and also increase the minimum at which spore and vegetative cell inocula of Cl. botulinum types A, B and E could initiate growth.