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Relationship between Heat Activation and Percentage Colony Formation for Bacillus stearothermophilus Spores: Effects of Storage and pH of the Recovery Medium
Author(s) -
Cook A. M.,
Brown M. R. W.
Publication year - 1965
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1965.tb02164.x
Subject(s) - spore , germination , microbiology and biotechnology , biology , food science , chemistry , botany
Colony counts of unheated spores were higher in a medium at pH 5·9 than in media of higher pH. The pattern was reversed as the spores were heated. Slide germination studies showed that about 8% or less of unheated spores germinated in slide culture. Optimal heat activation resulted in about 50% germination. The colony counts of heat activated spores dropped significantly upon storage for 9 months, but those of unheated spores did not.