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A Bacterial Spore Test Piece for the Control of Ethylene Oxide Sterilization
Author(s) -
Bebby Melanie M.,
Whitehouse C. E.
Publication year - 1965
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1965.tb02163.x
Subject(s) - spore , sterilization (economics) , ethylene oxide , aluminium foil , bacillus subtilis , foil method , chemistry , spore germination , bacterial spore , microbiology and biotechnology , materials science , biology , endospore , bacteria , composite material , organic chemistry , copolymer , polymer , genetics , layer (electronics) , monetary economics , economics , foreign exchange market , foreign exchange
Aluminium foil strips carrying varying numbers of spores of the Camp Detrick strain of Bacillus subtilis dried from water, 90% (v/v) methanol, 5 and 20% (v/v) serum, nutrient broth and isotonic saline were examined for their possible use as test pieces for the control of sterilization by ethylene oxide. Methanolic suspensions of these spores were found to be stable on storage, and foils carrying spores dried from methanol were the most reproducible and stable of those tested. The susceptibility of the test piece system could be controlled by varying the numbers of spores on each foil and the numbers of foils used per test.