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The Degradation of Gallic Acid and its Alkyl Esters by Pullularia pullulans
Author(s) -
Beveridge E. G.,
Hugo W. B.
Publication year - 1965
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1965.tb02157.x
Subject(s) - gallic acid , chemistry , levulinic acid , alkyl , metabolism , organic chemistry , oxygen , biochemistry , catalysis , antioxidant
By elective techniques an isolate of Pullularia pullulans was obtained which possessed the ability to utilize gallic acid (3,4,5‐trihydroxybenzoic acid) and its alkyl esters together with other aromatic substrates as the sole carbon source for growth in a mineral salts medium. Quantitative measurements were made of the disappearance of the gallates from culture media and of their metabolism by washed suspensions by the Warburg technique. The pattern of oxygen uptake with various substrates by suspensions adapted to gallic acid was examined in the light of Stanier's simultaneous adaptation postulates in an attempt to identify intermediates of gallic acid metabolism. As a result, β‐ketoadipic acid was suspected of being an intermediate. A keto compound was isolated as its 2,4‐dinitrophenylhydrazone during gallic acid metabolism but it was not β‐ketoadipic acid or levulinic acid.