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The Differentiation of Various Strains of Clostridium botulinum and Transformation Experiments with Type E
Author(s) -
Skulberg A.,
Hausken O. W.
Publication year - 1965
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1965.tb02129.x
Subject(s) - clostridium botulinum , transformation (genetics) , microbiology and biotechnology , spore , biology , strain (injury) , food science , clostridiaceae , genetics , toxin , gene , anatomy
The colonial forms and other characteristics of toxigenic and less toxigenic (hypotoxigenic) variants of Clostridium botulinum types A, B, C, D and E are described. In addition to differences in the appearance of the colonies the hypotoxigenic variants were able to produce larger numbers of spores. Transformation from one form to the other was found when they were grown in Robertson's meat broth to which sterile culture filtrates (boiled or unboiled) from the other form was added. A transformation from hypotoxigenic variants to toxigenic forms was also found when hypotoxigenic forms were grown in Robertson's medium to which the sterile culture filtrate (boiled or unboiled) of a strain of Cl. welchii had been added. The possibility of contamination of the cultures is emphasized. A final appraisal of the situation must be reserved until experiments with single cell isolates have been performed.