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The Maintenance of Discrete Bacterial Colonies on the Surface of Objects Embedded in Agar
Author(s) -
Kingston D.
Publication year - 1964
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1964.tb04917.x
Subject(s) - agar , agar plate , inoculation , bacteria , microbiology and biotechnology , chemistry , materials science , chromatography , biology , horticulture , genetics
Summary: Bacterial colonies grown on objects embedded in agar sometimes show a tendency to become confluent. Glass coverslips and polytetrafluorethylene (Teflon) discs were inoculated with Staphylococcus lactis and completely embedded in serum agar. If the surface of the agar were flooded with broth before the plates were incubated the colonies had a tendency to run together, but not if the plates were incubated after thorough drying. Drying also prevented confluence occurring when broth had been injected on to the surface of the embedded object. Drying the poured plate probably acts by removing fluid squeezed out at the interface.