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Studies on the Heat Resistance of Bacteria, with Particular Reference to the Genus Microbacterium : I. A New Technique using Solid Media
Author(s) -
Anagnostopoulos G. D.,
Seaman A.,
Woodbine M.
Publication year - 1964
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1964.tb04907.x
Subject(s) - petri dish , microbacterium , agar , base (topology) , agar plate , bacteria , heat resistance , surface (topology) , materials science , chemistry , microbiology and biotechnology , biology , composite material , mathematics , mathematical analysis , genetics , 16s ribosomal rna , geometry
Summary: The new technique depends upon spreading bacterial cells over the surface of an agar medium in a Petri dish. The cells are heated, cooled, grown and counted in situ . The plates are preheated on the surface of a water bath and held for the required time on the surface of a second bath at the desired temperature. They are cooled by cold water jetted on to the base. The experimental control of factors affecting the accuracy of the method are discussed and applications suggested.