Premium
The Use of the Wide‐Bore Dropping‐Mercury Electrode for the Determination of Rates of Oxygen Uptake and of Oxidation of Ammonia by Micro‐Organisms
Author(s) -
Painter H. A.,
Jones K.
Publication year - 1963
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1963.tb04797.x
Subject(s) - chemistry , oxygen , ammonia , environmental chemistry , respiration , polarography , nitrification , clark electrode , inorganic chemistry , mercury (programming language) , phenol , thiourea , activated sludge , electrode , environmental engineering , nitrogen , sewage treatment , environmental science , biochemistry , organic chemistry , botany , biology , computer science , electrolyte , programming language
S ummary : Convenient and reliable polarographic methods, using a wide‐bore dropping‐mercury electrode, for determining the dissolved oxygen content and the rate of respiration of sewage and activated sludge are described and verified by independent procedures. Using thiourea to inhibit Nitrosomonas spp., a technique was developed by which the uptake of oxygen by heterotrophs could be distinguished from that used in ammonia oxidation. Conditions are discussed under which accurate estimates of the rate of uptake of oxygen due to the oxidation of ammonia could be made. Typical values are given for the rates of respiration and of nitrification of activated sludge and also for the rates of respiration of a phenol‐destroying pseudomonad.