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A Genealogical Study of Clonal Development of Escherichia coli
Author(s) -
Quesnel L. B.
Publication year - 1963
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1963.tb04762.x
Subject(s) - escherichia coli , biology , alternation (linguistics) , population , inoculation , genetics , demography , gene , sociology , philosophy , immunology , linguistics
S ummary : The generation times of individual Escherichia coli cells inoculated on to a cellophane membrane surrounded by liquid synthetic medium in a continuous flow system were determined by direct observation for up to seven generations. The presence and production of nonviable cells was also recorded. A wide variety of developmental patterns was found among the developing clones; increased viability and a decrease in generation times with increasing age of the population were observed. The phenomenon of alternation of generations is described and discussed. Five factors contributory to the lag phase were distinguished, and their relative effect may be deduced from the results.