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THE RETENTION OF 32 P‐LABELLED RHIZOBIUM BY LEGUME SEED AFTER INOCULATION BY VACUUM TREATMENT
Author(s) -
COOPER R.
Publication year - 1962
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1962.tb01140.x
Subject(s) - rhizobium , legume , inoculation , bacteria , seed treatment , horticulture , biology , agronomy , botany , chemistry , germination , genetics
Legume seeds were vacuum treated in a suspension of 32 P‐labelled Rhizobium meliloti to find the numbers and uniformity of distribution of bacteria taken up and retained by the seeds after washing. Commercial seed retained 5–12 times as many bacteria as did hand harvested seed. From 5 to 15% of the seeds held 75–90% of the bacteria; vacuum treatment increased uptake by this small group of seeds by 60–300%, but had no significant effect on the majority that acquired relatively few bacteria. Therefore vacuum treatment of seeds during inoculation is likely to affect nodulation on a small proportion of the plants only.