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SPORULATION AND THE DEVELOPMENT OF RESISTANCE IN SPORING CULTURES OF CLOSTRIDIUM WELCHII
Author(s) -
CASH J. D.,
COLLEE J. G.
Publication year - 1962
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1962.tb01139.x
Subject(s) - spore , microbiology and biotechnology , heat resistance , endospore , biology , strain (injury) , clostridium , gram staining , bacteria , anatomy , genetics , materials science , composite material , antibiotics
The growth of a strain of Clostridium welchii type A in Ellner's medium was studied and the development of spores was correlated with the emergence of forms resistant to deleterious influences including heat, desiccation and thiomersalate. A qualitative increase in heat resistance was demonstrated, coincident with spore maturation over the course of 12 hr. The granular appearance of the bacterial cytoplasm prior to sporulation, as revealed by Gram's stain, precedes the development of acid‐fast material and does not seem to be associated with the presence of sudanophilic or metachromatic material.